Abstract
Replication-deficient adenoviruses are potent vaccine development platforms used extensively for human and animal candidate vaccines, largely due to their very good safety and immunogenicity profile. In this chapter we describe a method that can be used in any laboratory for the scalable production of replication-deficient adenovirus vector vaccines to GLP for preclinical studies in animal models, including definitive experimental studies in large target animal species for veterinary applications. We use human adenovirus serotype 5 (HAdV5) as an example, but the method can be easily adapted for use with other adenovirus serotypes from different species of origin.
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Acknowledgements
The Jenner Institute’s viral vector core facility and the Clinical Biomanufacturing Facility have optimized the above methods with funding from a Wellcome Trust strategic award.
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Morris, S.J., Turner, A.V., Green, N., Warimwe, G.M. (2016). Laboratory-Scale Production of Replication-Deficient Adenovirus Vectored Vaccines. In: Brun, A. (eds) Vaccine Technologies for Veterinary Viral Diseases. Methods in Molecular Biology, vol 1349. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3008-1_8
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DOI: https://doi.org/10.1007/978-1-4939-3008-1_8
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3007-4
Online ISBN: 978-1-4939-3008-1
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