On the substantial variation in serological responses in pigs to Sarcoptes scabiei var. suis using different commercially available indirect enzyme-linked immunosorbent assays

Parasitol Res. 2004 Sep;94(1):24-30. doi: 10.1007/s00436-004-1168-7. Epub 2004 Jul 28.

Abstract

Two trials were carried out to compare the use of different enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies against Sarcoptes scabiei var. suis. In the first trial, we employed four tests and 70 selected sera from a closed pig farm with sarcoptic mange. The sera were taken from the breeding area as well as from the finishing unit and compared with skin scrapings. The SARCOPTES-ELISA 2001 yielded the most positive results (88.58%), followed by the ELISA of the National Veterinary Institute, Uppsala (70%), the Acar-Test P-ELISA (52.86%), skin scrapings (48.57%), and the CHEKIT Sarcoptest (30%). In the second trial, eight litters from infected sows were examined from weeks 1-12 of life using the CHEKIT Sarcoptest and the SARCOPTES-ELISA 2001. The presence of maternal antibodies was highest on day 7 in both ELISAs and could be detected until weeks 5-9 of life. Antibodies increased as a result of an active immune response between days 56 and 63 in the SARCOPTES-ELISA 2001 and between days 70 and 77 of life in the CHEKIT Sarcoptest. Significant differences between the first and second litters were observed.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Antibodies / blood*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Female
  • Reagent Kits, Diagnostic*
  • Sarcoptes scabiei / immunology*
  • Scabies / immunology
  • Scabies / parasitology
  • Scabies / veterinary*
  • Sensitivity and Specificity
  • Swine
  • Swine Diseases / diagnosis*
  • Swine Diseases / immunology
  • Swine Diseases / parasitology

Substances

  • Antibodies
  • Reagent Kits, Diagnostic